Research Technician III, Cellular and Molecular Medicine (Full-Time)

Perform complex experimental procedures, including: Growth of human endothelial cell cultures in single and pooled cultures. Exposing cultured cells to shear stress to mimic hemodynamic blood flow.

Performing molecular biology techniques such as nucleic acid extractions, quantitative real-time PCR, RNA-seq, ATAC-seq, ChIP-seq, and other -seq assays including library preparation. Performing immunolabeling, immunoblotting, and confocal/high-content microscopy for cellular imaging. Transfect/infect cells with oligos and lentivirus in vitro for gain- and loss-of-function experiments.

Protocol Design and Refinements

With minimal supervisor guidance, refines, adapts, and makes decisions to switch experiment parameters in response to situational demands of experiments. Assists in preparing experimental designs, technical protocols and techniques, and Standard Operating Procedures (SOPs). Record-keeping, Analysis, and Presentations:

Communicates effectively all aspects of experiments with PI or other lab members and collaborators. Keeps accurate and detailed records of experiments, results, and enters data in laboratory databases and filesystems. Communicates with collaborating investigators and laboratories.

Catalogs specimens and samples. Performs independent analysis and interpretation of data for investigator and team.

Operational Support

Orders and catalogs laboratory supplies and supports laboratory operations. Assist in maintaining laboratory equipment and related records. Operates and calibrates specialized scientific equipment including instruments for exposing cell cultures to fluid shear stress.

Knowledge, Skills Abilities: Ability to maintain protocols, data, and samples organized with clear documentation recording how experiments were performed to ensure rigor and reproducibility. Ability to communicate clearly with all members of the team.