Research Technician II

Maintain and monitor mouse colony in sterile vivarium in accordance with laboratory and IACUC standards including: o Breeding, timely replacement of breeding mice, and weaning. o Mouse identification via ear tagging, toe tipping, and genotyping PCR Execute protocols related to protein preparation and purification including site-directed mutagenesis, bacterial cell transformation, and column purification.

Perform administrative support tasks such as: o Tracking inventory and ordering supplies in accordance with university purchasing policies o Clean, sterilize, and maintain laboratory equipment o Organize laboratory equipment and prepare standard chemical stocks and buffers Develop, write, and implement standard operating protocols including western blotting, DNA/RNA isolation, and mouse tissue processing. Analyze data for the investigator and prepare summary reports for weekly lab meetings. Keep an accurate and detailed record of experiments and results.

Knowledge, Skills, and Abilities (KSAs): Knowledge of the principles and techniques of the subject discipline. Knowledge of modern research methods, data collection and analysis and skill in their application.

Skill in analyzing and evaluating data. Ability to effectively communicate.